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KMID : 0613820180280030331
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Journal of Life Science
2018 Volume.28 No. 3 p.331 ~ p.338
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Effect of Rhynchosia Nulubilis Ethanolic Extract on DOPA Oxidation and Melanin Synthesis
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Kim Jae-Ryeon
Kim Moon-Moo
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Abstract
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Melanin is a polymer substance that plays an important role in the determination of hair growth and skin color in vivo. However, melanin, which is over-produced by reactive oxygen species, is known to cause stains, freckles, and hypercholesterolemia, which are associated with aging. Previous studies have shown that polyphosphate, one of the components of Rhynchosia Nulubilis, inhibits skin aging induced by ultraviolet rays. The aim of this study is to investigate the direct effect of Rhynchosia Nulubilis ethanolic extract (RNEE) on melanin synthesis. In this study, RNEE showed no antioxidative effects on scavenging activity of DPPH radical in addition to reducing power. The cytotoxicity of RNEE was increased in a dose-dependent manner in an MTT assay. In addition, RNEE increased tyrosinase activity and melanin synthesis in DOPA-oxidation experiments. RNEE did not promote the conversion L-DOPA into melanin in live cells, but melanin production was promoted in the RNEE-treated group after H2O2 pretreatment compared to the control group in which melanin production was reduced by treatment with H2O2. In addition, RNEE increased the expression level of tyrosinase related protein-2 (TRP-2) and increased the expression level of tyrosinase related protein-1 (TRP-1) at a concentration of 16 μg/ml. In particular, it was found that RNEE increased the expression level of SOD-3, by which superoxide anion is converted to hydrogen peroxide, higher than the control and α-MSH used as a positive control at a concentration of more than 16 μg/ml. The results suggest that RNEE can induce melanogenesis related to black hair.
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KEYWORD
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Melanin, Rhynchosia Nulubilis, tyrosinase, TRP-1, TRP-2
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